| Gene | Ifitm1 |
| Chromosome | 7 |
| Deletion Start | 140,969,692 |
| Deletion End | 140,968,259 |
| Deletion Size | 1,434 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,955,718 | 140,955,741 | TGGTCCCTGTTCAATACACTCTTC |
| TUP | 140,968,392 | 140,968,415 | TGAACTTCTGCTGCCTGGGCTTCG |
| TUR | 140,968,444 | 140,968,465 | AAGTCAAGGTTGAGGCCCATAC |
| TDF | 140,969,326 | 140,969,346 | GCTGGGTGTTTCTGGGTAGTC |
| TDP | 140,969,357 | 140,969,382 | TAAAGTGGAAGAAGGTCCCTGGCCTA |
| TDR | 140,969,399 | 140,969,419 | CATCACCTTCCTAGCCCACTG |
PCR Primers
| Primer | Sequence |
| SU | GAACACTGCAGTCACCTCTC |
| SD | GACATGAAGCCAGAAGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 12748 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17126A-B4 | VGB6 | C57BL/6NTac | |
| 17126A-B8 | VGB6 | C57BL/6NTac | |
| 17126A-B10 | VGB6 | C57BL/6NTac | |
| 17126A-G7 | VGB6 | C57BL/6NTac | |
| 17126A-D3 | VGB6 | C57BL/6NTac | |
