| Gene | Ifitm6 |
| Chromosome | 7 |
| Deletion Start | 141,016,855 |
| Deletion End | 141,015,902 |
| Deletion Size | 954 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,016,724 | 141,016,743 | ATGGCTGCTGCCTGGGTTTC |
| TUP | 141,016,696 | 141,016,721 | TGCCTACATCTACTCGGTGAAGGTGA |
| TUR | 141,016,659 | 141,016,679 | GGAGGAGCCACAACCACATAC |
| TDF | 141,016,179 | 141,016,196 | TGCTCCAAGGCCACACTG |
| TDP | 141,016,148 | 141,016,174 | TTTCATCTCTTTCAGTCCAGGGACCGG |
| TDR | 141,016,101 | 141,016,120 | GCGGTTGAAGCATGGGATTG |
PCR Primers
| Primer | Sequence |
| SU | GCCCTTCTTGAACCCTATCC |
| SD | ATGCCCTGCACTATACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17127A-D5 | VGB6 | C57BL/6NTac | |
| 17127A-G5 | VGB6 | C57BL/6NTac | |
| 17127A-C8 | VGB6 | C57BL/6NTac | |
| 17127A-E11 | VGB6 | C57BL/6NTac | |
| 17127A-D7 | VGB6 | C57BL/6NTac | |
| 17127A-D8 | VGB6 | C57BL/6NTac | |
