| Gene | Il2rb |
| Chromosome | 15 |
| Deletion Start | 78,491,846 |
| Deletion End | 78,481,406 |
| Deletion Size | 10,441 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,491,623 | 78,491,641 | GGACCCAAGGGCAGGTATC |
| TUP | 78,491,597 | 78,491,622 | CATCTTCCTGAGCTTGCACAGGCTTC |
| TUR | 78,491,532 | 78,491,552 | GAAGACACAGAGCGCCTATCC |
| TDF | 78,481,838 | 78,481,857 | TCTGGCTGGAGAACAGGATG |
| TDP | 78,481,815 | 78,481,837 | ACTACTGTGCCTTCCCGCCCAGG |
| TDR | 78,481,793 | 78,481,811 | GGGAGAAGAGCAGCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGCTGTCCAATCACAGAG |
| SD | GGGCAGAGGGAAGTAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17130D-B10 | VGB6 | C57BL/6NTac | |
| 17130D-E5 | VGB6 | C57BL/6NTac | |
