| Gene | Il4i1 |
| Chromosome | 7 |
| Deletion Start | 44,840,707 |
| Deletion End | 44,836,528 |
| Deletion Size | 4,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,837,177 | 44,837,198 | TTAAGCTGGGAAGTCCCTCTAC |
| TUP | 44,837,201 | 44,837,228 | TCCATCTGCTAGTCTTCCTGCTACAGTC |
| TUR | 44,837,233 | 44,837,252 | GCTGCTCGGAAAGAAAGGTC |
| TDF | 44,840,437 | 44,840,459 | AAGAAGTCAGCCCAGATGAACAG |
| TDP | 44,840,461 | 44,840,487 | AGCCCTCTCACAAACACTTGTTGGTGG |
| TDR | 44,840,516 | 44,840,534 | GGGAATGGCCTCCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGCATCTGACCCTAGCTC |
| SD | CTCCTAGAGGTCCGGGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17131A-A2 | VGB6 | C57BL/6NTac | |
| 17131A-B7 | VGB6 | C57BL/6NTac | |
| 17131A-H10 | VGB6 | C57BL/6NTac | |
| 17131A-D9 | VGB6 | C57BL/6NTac | |
