| Gene | Inhbb |
| Chromosome | 1 |
| Deletion Start | 119,421,083 |
| Deletion End | 119,417,288 |
| Deletion Size | 3,796 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,420,838 | 119,420,855 | TTCCTGGAGGCGGTGAAG |
| TUP | 119,420,815 | 119,420,837 | AGACACATCTTGAGCCGCCTGCA |
| TUR | 119,420,781 | 119,420,799 | GACAGCGTGCGTGATGTTG |
| TDF | 119,417,616 | 119,417,636 | TGCAGGCAACAGTTCTTCATC |
| TDP | 119,417,590 | 119,417,613 | CTTTCGGCTCATCGGCTGGAACGA |
| TDR | 119,417,555 | 119,417,574 | AGTTCCCGTAGTAGCCAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTGCAGATGCCTAG |
| SD | TTCAGACTGACAGGCATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17132B-F11 | VGB6 | C57BL/6NTac | |
| 17132B-D10 | VGB6 | C57BL/6NTac | |
| 17132B-B10 | VGB6 | C57BL/6NTac | |
| 17132C-C2 | VGB6 | C57BL/6NTac | |
| 17132C-F11 | VGB6 | C57BL/6NTac | |
