| Gene | Inhbc |
| Chromosome | 10 |
| Deletion Start | 127,370,393 |
| Deletion End | 127,356,990 |
| Deletion Size | 13,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,369,758 | 127,369,776 | CAGGCCAAGGGTCGATTAC |
| TUP | 127,369,730 | 127,369,757 | TGGGCAGTTTGTGATTTCTCTGACTCCA |
| TUR | 127,369,702 | 127,369,725 | CGAGGAAGAGGAGGTGATTGATAG |
| TDF | 127,357,856 | 127,357,876 | CACTCCAAAGGCTGCGATAAC |
| TDP | 127,357,826 | 127,357,854 | TCCCTGTGGCTTCTGATGTTTACAGACCT |
| TDR | 127,357,801 | 127,357,819 | GAGCCGGGTCTGGTTGATG |
PCR Primers
| Primer | Sequence |
| SU | CTTTCACTGGCTACAGGAG |
| SD | AGCCTTTCTTTGGAAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17133A-H4 | VGB6 | C57BL/6NTac | |
| 17133A-B3 | VGB6 | C57BL/6NTac | |
| 17133A-B7 | VGB6 | C57BL/6NTac | |
| 17133A-G11 | VGB6 | C57BL/6NTac | |
| 17133A-G4 | VGB6 | C57BL/6NTac | |
| 17133A-F6 | VGB6 | C57BL/6NTac | |
