| Gene | Itfg3 |
| Chromosome | 17 |
| Deletion Start | 26,220,545 |
| Deletion End | 26,213,465 |
| Deletion Size | 7,081 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,220,345 | 26,220,366 | CGTGGTGTTTGTCCTGTCTTTC |
| TUP | 26,220,321 | 26,220,343 | TCATCCCATGTCCAGACCGTCCC |
| TUR | 26,220,274 | 26,220,294 | GGCTCACCTGCATTGTTGTAG |
| TDF | 26,213,674 | 26,213,692 | TGCTGCCTATGTGCTCCTG |
| TDP | 26,213,650 | 26,213,672 | CGGGCCCAGCAAGCTCAGATGTG |
| TDR | 26,213,610 | 26,213,630 | GGTCTTGCACTTTGTGTTTGG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGACAGGACAGGAGTGC |
| SD | GAGGGTAAGGAGGCAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17134A-B11 | VGB6 | C57BL/6NTac | |
| 17134C-H5 | VGB6 | C57BL/6NTac | |
| 17134C-G4 | VGB6 | C57BL/6NTac | |
