| Gene | Itga3 |
| Chromosome | 11 |
| Deletion Start | 95,076,333 |
| Deletion End | 95,046,391 |
| Deletion Size | 29,943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,075,622 | 95,075,639 | AACCAGGCTGGACCACAC |
| TUP | 95,075,598 | 95,075,620 | TCTCAAGGGCTTGGCATTGCAGA |
| TUR | 95,075,555 | 95,075,575 | GTCTCTGGACAGCTCCGAATG |
| TDF | 95,046,541 | 95,046,563 | CGTGCCCTGTATGAAGCTAAGAG |
| TDP | 95,046,508 | 95,046,532 | CTGAGATGAAGAGCCAGCCGTCAGA |
| TDR | 95,046,486 | 95,046,503 | GTCGTCGGTCAGCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGGTGATCGAGGGCTTG |
| SD | ATTGTGGACCAGGAAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17136A-A8 | VGB6 | C57BL/6NTac | |
| 17136A-B3 | VGB6 | C57BL/6NTac | |
| 17136A-G8 | VGB6 | C57BL/6NTac | |
| 17136A-F8 | VGB6 | C57BL/6NTac | |
| 17136A-E11 | VGB6 | C57BL/6NTac | |
| 17136A-H2 | VGB6 | C57BL/6NTac | |
