| Gene | Itm2c |
| Chromosome | 1 |
| Deletion Start | 85,907,701 |
| Deletion End | 85,894,667 |
| Deletion Size | 13,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,895,362 | 85,895,383 | TGAGCCTTCTCCCATTTCACAC |
| TUP | 85,895,385 | 85,895,409 | TGCGGGCCCTACTTCTTAGCTCCTT |
| TUR | 85,895,430 | 85,895,450 | GCCCACTCAGCAGATGGTAAC |
| TDF | 85,907,378 | 85,907,399 | CATGACAGGCACAGTATGAGAC |
| TDP | 85,907,400 | 85,907,421 | TGACCTAGGCGCTCCCTCTACC |
| TDR | 85,907,454 | 85,907,473 | GCGAGGCAAGTGAGGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | GAGGAGGGAGATGCAGTTTG |
| SD | AAAGTCTTTCAGGGCTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17142A-B1 | VGB6 | C57BL/6NTac | |
| 17142A-F12 | VGB6 | C57BL/6NTac | |
