| Gene | Kcne2 |
| Chromosome | 16 |
| Deletion Start | 92,297,041 |
| Deletion End | 92,296,590 |
| Deletion Size | 452 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,296,721 | 92,296,745 | CTTCTACTACGTCATCCTGTACCTC |
| TUP | 92,296,753 | 92,296,779 | TGATCGGCATGTTCTCGTTCATCGTGG |
| TUR | 92,296,788 | 92,296,806 | ACTTCACCGTGCTCACCAG |
| TDF | 92,296,822 | 92,296,841 | ACTCCCAGGACCCGTACCAC |
| TDP | 92,296,846 | 92,296,875 | ACATCGTGGAAGATTGGCAGGAAAAGTACA |
| TDR | 92,296,890 | 92,296,910 | GGCCTTGGAGTCTTCCAGATG |
PCR Primers
| Primer | Sequence |
| SU | AACTTTATGCTTTGAGGCTGC |
| SD | TCGCTGCCCATACATAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17143A-D5 | VGB6 | C57BL/6NTac | |
| 17143A-H5 | VGB6 | C57BL/6NTac | |
| 17143A-A9 | VGB6 | C57BL/6NTac | |
| 17143A-A10 | VGB6 | C57BL/6NTac | |
| 17143A-F12 | VGB6 | C57BL/6NTac | |
| 17143A-E11 | VGB6 | C57BL/6NTac | |
