| Gene | Kcnh3 |
| Chromosome | 15 |
| Deletion Start | 99,242,541 |
| Deletion End | 99,224,979 |
| Deletion Size | 17,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,225,398 | 99,225,415 | AGGCGGGATTCAGCAGAG |
| TUP | 99,225,419 | 99,225,441 | TGTGTCTGCAAGCGTATCGCACA |
| TUR | 99,225,442 | 99,225,460 | CCAAGCGACGGGTTTAGAC |
| TDF | 99,242,463 | 99,242,481 | ATTGGCTGCCACGGTCCTG |
| TDP | 99,242,483 | 99,242,504 | CACGGTCCAGTGGACCCAGGAA |
| TDR | 99,242,519 | 99,242,537 | CATCTCCAGCCGCAGTCAG |
PCR Primers
| Primer | Sequence |
| SU | CGACAGACGGAGGGAGCTG |
| SD | CCACCATTGTCACTCCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17146A-C6 | VGB6 | C57BL/6NTac | |
| 17146A-A5 | VGB6 | C57BL/6NTac | |
