| Gene | Kcnj5 |
| Chromosome | 9 |
| Deletion Start | 32,323,014 |
| Deletion End | 32,317,499 |
| Deletion Size | 5,516 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,322,592 | 32,322,615 | GGCTTTGTATCTGCTTTCCTGTTC |
| TUP | 32,322,559 | 32,322,586 | CGAGACAGAAACAACCATTGGGTATGGC |
| TUR | 32,322,515 | 32,322,538 | AGGAGTATGATCCCTTCTGGACAC |
| TDF | 32,317,893 | 32,317,915 | TCTGTTTCTCCAGGCATGACTTG |
| TDP | 32,317,865 | 32,317,892 | CCAAGCACGGAGCTCTTACATGGATACA |
| TDR | 32,317,831 | 32,317,851 | GAACTGGTGTGAATCGGTGAC |
PCR Primers
| Primer | Sequence |
| SU | ATATTAGAAATCCACATGAGG |
| SD | CAGCTGGAGGCCTGAGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17149A-H5 | VGB6 | C57BL/6NTac | |
