| Gene | Lgi4 |
| Chromosome | 7 |
| Deletion Start | 31,069,193 |
| Deletion End | 31,060,024 |
| Deletion Size | 9,170 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,060,157 | 31,060,175 | TGCCTGAGAGCTTCTCCAC |
| TUP | 31,060,177 | 31,060,200 | ACGCTCCTGTCACTGTGAGTGCCA |
| TUR | 31,060,205 | 31,060,224 | CTCCCTTTCGGCCCACATAG |
| TDF | 31,068,667 | 31,068,686 | ACGGGCATAGTGTTGGTGTC |
| TDP | 31,068,688 | 31,068,711 | CAGAGGCCTGTCATCTCAGCACCA |
| TDR | 31,068,726 | 31,068,746 | CCTGGCACTGACTGATCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TAAAGGTCAAACCAGAGAGAC |
| SD | TGTGTACCAGCCTCAACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17152A-C10 | VGB6 | C57BL/6NTac | |
| 17152A-E10 | VGB6 | C57BL/6NTac | |
