| Gene | Lrrc59 |
| Chromosome | 11 |
| Deletion Start | 94,643,573 |
| Deletion End | 94,630,008 |
| Deletion Size | 13,566 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,630,587 | 94,630,609 | CAGGACTGGCAAAGGTTCTCATC |
| TUP | 94,630,610 | 94,630,634 | CCAGCTGCTCTGAAACTGTCCGGAA |
| TUR | 94,630,672 | 94,630,691 | GGGCCTCAGGCTTCCAAATG |
| TDF | 94,643,448 | 94,643,469 | CACCAGCGTGAACACCATCTAC |
| TDP | 94,643,471 | 94,643,491 | ACAATGCGGTCCAGGGCCTGC |
| TDR | 94,643,500 | 94,643,520 | CAGGACCCACTGGAGGATCTC |
PCR Primers
| Primer | Sequence |
| SU | GATGCAAACACAGGTCATGG |
| SD | GCCTATGCCTTCTCTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17155A-G3 | VGB6 | C57BL/6NTac | |
| 17155A-F7 | VGB6 | C57BL/6NTac | |
| 17155A-E12 | VGB6 | C57BL/6NTac | |
| 17155A-C7 | VGB6 | C57BL/6NTac | |
