| Gene | Lrtm2 |
| Chromosome | 6 |
| Deletion Start | 119,323,712 |
| Deletion End | 119,317,040 |
| Deletion Size | 6,673 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,323,238 | 119,323,259 | CAGCGGGATCTGTATGCTTCAG |
| TUP | 119,323,212 | 119,323,233 | CCCAGCCCTGTAGCGGCACTAG |
| TUR | 119,323,187 | 119,323,205 | CCCACTGCTGCACCCTAAG |
| TDF | 119,317,826 | 119,317,847 | GATGTACCAAAGCCATGTCCTG |
| TDP | 119,317,791 | 119,317,817 | CCCAGCTGTTACTGCTGTAGGTCAGTG |
| TDR | 119,317,768 | 119,317,786 | TCAAGCCTGTCCCTCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TGACGGGACTGGTACATCTA |
| SD | GGCCCTAATGCAGACTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17156A-H3 | VGB6 | C57BL/6NTac | |
| 17156A-E1 | VGB6 | C57BL/6NTac | |
| 17156A-G11 | VGB6 | C57BL/6NTac | |
| 17156A-F1 | VGB6 | C57BL/6NTac | |
| 17156A-G4 | VGB6 | C57BL/6NTac | |
