| Gene | Mall |
| Chromosome | 2 |
| Deletion Start | 127,729,863 |
| Deletion End | 127,705,812 |
| Deletion Size | 24,052 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,728,968 | 127,728,988 | TTCTTGGGAAGCGTCCTTGTG |
| TUP | 127,728,946 | 127,728,965 | TCCCACAGGTGGGTGGCCTT |
| TUR | 127,728,922 | 127,728,945 | TGCCTCTTAGGTGGAGTCTCTATC |
| TDF | 127,706,022 | 127,706,041 | GGAGCTGGACAACTGGAAAC |
| TDP | 127,705,999 | 127,706,021 | TGTAGCCCTAGCAACGCCCAAGG |
| TDR | 127,705,944 | 127,705,963 | AGGGTCAGTGCGGTGCAATC |
PCR Primers
| Primer | Sequence |
| SU | GCTCCAGCATTCAAAGTTCC |
| SD | GTGTGCAAGGCCAGTAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17160C-B2 | VGB6 | C57BL/6NTac | |
| 17160C-G5 | VGB6 | C57BL/6NTac | |
| 17160C-B4 | VGB6 | C57BL/6NTac | |
