| Gene | Mrpl18 |
| Chromosome | 17 |
| Deletion Start | 12,915,995 |
| Deletion End | 12,911,631 |
| Deletion Size | 4,365 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,915,066 | 12,915,084 | GAGGACCCAGGCAGCTTAC |
| TUP | 12,915,036 | 12,915,063 | TGGTCTGTAATTCCAGTTCCCAGGGATC |
| TUR | 12,915,014 | 12,915,034 | CCAAGGTCAGAAGAGGGTGTC |
| TDF | 12,912,037 | 12,912,058 | TTTGGTCAGACATGGTTGGTTC |
| TDP | 12,912,010 | 12,912,034 | TCCTAGGTCTTGGTCCAGCCTCTGG |
| TDR | 12,911,963 | 12,911,982 | TGCCATGAGAGGAAGCCTAC |
PCR Primers
| Primer | Sequence |
| SU | TAGGGATTGACAGACGAGGG |
| SD | TCACAGAACTCCACTCGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17161A-E8 | VGB6 | C57BL/6NTac | |
| 17161A-A12 | VGB6 | C57BL/6NTac | |
| 17161A-H2 | VGB6 | C57BL/6NTac | |
