| Gene | Mrpl27 |
| Chromosome | 11 |
| Deletion Start | 94,659,878 |
| Deletion End | 94,653,805 |
| Deletion Size | 6,074 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,653,882 | 94,653,900 | GAGGCTGGGAGATGGAAAC |
| TUP | 94,653,901 | 94,653,927 | TGGAGCCCATGGTGAACCTAGATACGG |
| TUR | 94,653,951 | 94,653,970 | GAGACAGTCGCCCTCCAAAG |
| TDF | 94,659,336 | 94,659,353 | TTGTGGGAGCCTGGCTTG |
| TDP | 94,659,358 | 94,659,381 | TCTGCCCACTGTAGCTTCTGAGCA |
| TDR | 94,659,385 | 94,659,404 | AACAGCAGTAACCGCAGCAC |
PCR Primers
| Primer | Sequence |
| SU | AGCACAGAATCTAGCTGAGC |
| SD | TGACCCTGAAGAGCAGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17162A-A4 | VGB6 | C57BL/6NTac | |
| 17162A-H10 | VGB6 | C57BL/6NTac | |
| 17162A-B4 | VGB6 | C57BL/6NTac | |
| 17162A-G12 | VGB6 | C57BL/6NTac | |
| 17162A-F1 | VGB6 | C57BL/6NTac | |
| 17162A-F5 | VGB6 | C57BL/6NTac | |
