| Gene | Mrpl54 |
| Chromosome | 10 |
| Deletion Start | 81,266,887 |
| Deletion End | 81,264,796 |
| Deletion Size | 2,092 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,266,435 | 81,266,453 | TTGCCTGGAAAGCCTTGAG |
| TUP | 81,266,405 | 81,266,427 | CCCAGGACCCACATAAGCAGGGA |
| TUR | 81,266,376 | 81,266,395 | CACAGTGCTTGTGGGATTGG |
| TDF | 81,264,910 | 81,264,929 | GGCGACTACTTCGCAAACAG |
| TDP | 81,264,881 | 81,264,900 | CGCCACAACCGGCTGAGCAA |
| TDR | 81,264,849 | 81,264,870 | GGTGCCCATCATCACACTACAG |
PCR Primers
| Primer | Sequence |
| SU | CGTCATCATCGTCTGAGG |
| SD | AGTGAGCTGCACCCACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17163A-A8 | VGB6 | C57BL/6NTac | |
| 17163A-A12 | VGB6 | C57BL/6NTac | |
| 17163A-H12 | VGB6 | C57BL/6NTac | |
| 17163A-D12 | VGB6 | C57BL/6NTac | |
| 17163A-E10 | VGB6 | C57BL/6NTac | |
| 17163A-G4 | VGB6 | C57BL/6NTac | |
