| Gene | Mrps5 |
| Chromosome | 2 |
| Deletion Start | 127,603,873 |
| Deletion End | 127,587,431 |
| Deletion Size | 16,443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,587,971 | 127,587,988 | GCTTCGCGCTGGACATTG |
| TUP | 127,587,989 | 127,588,013 | TGCTCAAGGTCCTGGTCCAGAAAGT |
| TUR | 127,588,045 | 127,588,069 | GGCTCAAGTCACATACCCTATCAAG |
| TDF | 127,602,949 | 127,602,969 | GGTGCGGTTACTCTGGTGTAG |
| TDP | 127,602,971 | 127,602,995 | CCTGGAAGCAAAGAGTGCCATGTCC |
| TDR | 127,603,001 | 127,603,019 | CAACCCAGGGTCCCATCTC |
PCR Primers
| Primer | Sequence |
| SU | TCAAGCCTGCTACTGGTGAG |
| SD | ACAGAAACGGCACACAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17164A-D12 | VGB6 | C57BL/6NTac | |
| 17164A-C11 | VGB6 | C57BL/6NTac | |
| 17164A-A3 | VGB6 | C57BL/6NTac | |
| 17164A-E1 | VGB6 | C57BL/6NTac | |
