| Gene | Npr1 |
| Chromosome | 3 |
| Deletion Start | 90,465,500 |
| Deletion End | 90,451,116 |
| Deletion Size | 14,385 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,464,877 | 90,464,896 | TGCGTGAGCGACTCAACATC |
| TUP | 90,464,847 | 90,464,872 | TAAATCACCAGGAGTTCGTCGAGGGC |
| TUR | 90,464,816 | 90,464,835 | TCCGCAGTAGCTTGGTGTAG |
| TDF | 90,451,729 | 90,451,749 | CCCTACCTACCGCACAGAAAC |
| TDP | 90,451,707 | 90,451,728 | CAGACTGCTGCACATGGAGGCA |
| TDR | 90,451,683 | 90,451,705 | CTGACTTTGACCTCCTGACCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTCGCTCTTCTTAGATCGCC |
| SD | TTCTCTTTCCTTCAGGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17166A-B10 | VGB6 | C57BL/6NTac | |
| 17166A-E2 | VGB6 | C57BL/6NTac | |
| 17166A-E3 | VGB6 | C57BL/6NTac | |
| 17166A-F10 | VGB6 | C57BL/6NTac | |
| 17166A-H10 | VGB6 | C57BL/6NTac | |
| 17166A-G2 | VGB6 | C57BL/6NTac | |
