| Gene | Pla2g10 |
| Chromosome | 16 |
| Deletion Start | 13,730,408 |
| Deletion End | 13,715,434 |
| Deletion Size | 14,975 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,730,091 | 13,730,111 | CCCATAGTTACTGCGGTCCTC |
| TUP | 13,730,068 | 13,730,090 | TCCAGCAGCCTGAAGCTCCTAGC |
| TUR | 13,730,033 | 13,730,055 | AAGTGATAGCCAGTTCACATGAC |
| TDF | 13,715,619 | 13,715,640 | CCTCTGAGTCTCTGCTCTAACC |
| TDP | 13,715,587 | 13,715,614 | TTTCCATACTTCTTCCAGGACCAGCAGA |
| TDR | 13,715,541 | 13,715,559 | AGCCAGCTCCTCGTCACAC |
PCR Primers
| Primer | Sequence |
| SU | AGCGTCAGCAGCATCTTTAC |
| SD | CCAAGCCTAAGCAGGTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17168A-A4 | VGB6 | C57BL/6NTac | |
| 17168A-B4 | VGB6 | C57BL/6NTac | |
| 17168A-C9 | VGB6 | C57BL/6NTac | |
| 17168A-E4 | VGB6 | C57BL/6NTac | |
| 17168A-F5 | VGB6 | C57BL/6NTac | |
| 17168A-D4 | VGB6 | C57BL/6NTac | |
