17172   Prss45     Status: ES cell colonies screened / QC positives

GenePrss45
Chromosome9
Deletion Start110,841,170
Deletion End110,834,637
Deletion Size6,534
Genome Build38
MGI IDMGI:3605764
NCBI Gene ID260408
BAC ID392O4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 9

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF110,834,700110,834,717TTTGCGGCGCTACTGTTG
TUP110,834,719110,834,738TGCCTCCGCGGCCAAACTTA
TUR110,834,750110,834,773GAAAGGACAATGGAGAAGGAGGTG
TDF110,840,739110,840,759GCACTCCGGGAGAAATGATTC
TDP110,840,761110,840,786TTCAAGCAGAACAGAACGGAGAGGGT
TDR110,840,827110,840,847TCCCTGGAGAAGACAAAGGAC

PCR Primers

PrimerSequence
SUTTGTGCAACAGGCTGAGAAC
SDTACTGCCACTGTTGACCCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR109 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17172A-A3VGB6C57BL/6NTac
17172A-F6VGB6C57BL/6NTac
17172A-C12VGB6C57BL/6NTac
17172A-E12VGB6C57BL/6NTac
17172A-H11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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