| Gene | Sema6a |
| Chromosome | 18 |
| Deletion Start | 47,306,452 |
| Deletion End | 47,276,591 |
| Deletion Size | 29,862 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,306,385 | 47,306,406 | GGGCTGGTTTCCCAGAAGATTC |
| TUP | 47,306,359 | 47,306,384 | CGAGCCAATCAGTATTTCGCATGGCA |
| TUR | 47,306,296 | 47,306,316 | GGGCTGATGCAGGCTAAGATG |
| TDF | 47,276,722 | 47,276,744 | GACAAGCAGGAAGTGACAAAGAC |
| TDP | 47,276,696 | 47,276,720 | TCTGAGTGTTGGGCTCCCACATTGA |
| TDR | 47,276,670 | 47,276,688 | AGCAGCGATGAGCCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCCTGTAATGGTCTGGTC |
| SD | CTGTCTCATGTAAGCAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 111 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17174A-G8 | VGB6 | C57BL/6NTac | |
| 17174A-E7 | VGB6 | C57BL/6NTac | |
