| Gene | Serpini1 |
| Chromosome | 3 |
| Deletion Start | 75,640,768 |
| Deletion End | 75,613,101 |
| Deletion Size | 27,668 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,613,106 | 75,613,125 | CCTTGAACTGCTTGCTTTGC |
| TUP | 75,613,126 | 75,613,148 | TGGCCTTGCAAAGTGTGGTGACA |
| TUR | 75,613,164 | 75,613,187 | TGACCACTCAGTTATGGTTTCATC |
| TDF | 75,639,869 | 75,639,891 | GACCTGCCTGTCCTGTTTCTAAC |
| TDP | 75,639,895 | 75,639,919 | ACAGGCTGCCTTGTCTGCTTCTCAG |
| TDR | 75,639,950 | 75,639,969 | ACCCACCCTGGCATGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | AAATGTTTAGTGTGTGTGCCTTC |
| SD | TGCTTACTTTGGTGTTTGATTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17176B-C6 | VGB6 | C57BL/6NTac | |
| 17176C-B8 | VGB6 | C57BL/6NTac | |
| 17176C-C12 | VGB6 | C57BL/6NTac | |
| 17176C-D6 | VGB6 | C57BL/6NTac | |
| 17176C-H2 | VGB6 | C57BL/6NTac | |
