| Gene | Siglec5 |
| Chromosome | 7 |
| Deletion Start | 43,358,864 |
| Deletion End | 43,351,551 |
| Deletion Size | 7,314 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,352,315 | 43,352,335 | ACAGGACCACCCTCTCCTCAG |
| TUP | 43,352,336 | 43,352,357 | AGCTGAACCTCACACCCAGGCC |
| TUR | 43,352,394 | 43,352,416 | CAGTAACACCAGTGCCAGGTAAG |
| TDF | 43,358,519 | 43,358,543 | CTTTGGTAATTCTTGCTGCCCTCTG |
| TDP | 43,358,552 | 43,358,581 | TGACTGATTCCCTTCTCTAATCTGGTTCCA |
| TDR | 43,358,607 | 43,358,627 | TGGCTGTTCTTTCTGGGTCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGTTCAGGAGTGGAGGGTG |
| SD | ATGTAGCTACAACACAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17178A-B9 | VGB6 | C57BL/6NTac | |
| 17178A-D2 | VGB6 | C57BL/6NTac | |
| 17178A-E5 | VGB6 | C57BL/6NTac | |
| 17178A-G5 | VGB6 | C57BL/6NTac | |
| 17178A-G12 | VGB6 | C57BL/6NTac | |
| 17178A-F5 | VGB6 | C57BL/6NTac | |
