| Gene | Siglece |
| Chromosome | 7 |
| Deletion Start | 43,660,129 |
| Deletion End | 43,651,456 |
| Deletion Size | 8,674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,659,246 | 43,659,266 | TGACCCTGCCTGGAACTAATG |
| TUP | 43,659,219 | 43,659,245 | TGTCCACAAGAATGACCATCCGTCTCA |
| TUR | 43,659,197 | 43,659,216 | AGCCCAGCCTCACATGACAC |
| TDF | 43,652,096 | 43,652,119 | CGTCTCGGCTTCACATCTATAAAC |
| TDP | 43,652,066 | 43,652,094 | AGAGACAGAAGGACTATCTTGCTGGTGGA |
| TDR | 43,652,001 | 43,652,024 | GGAATCACCTTCACTCCTACTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTAAAGGAAGGAGGGATGC |
| SD | GTCAAGTGCTGGGTGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17179A-H11 | VGB6 | C57BL/6NTac | |
| 17179A-C4 | VGB6 | C57BL/6NTac | |
| 17179A-E4 | VGB6 | C57BL/6NTac | |
| 17179A-G10 | VGB6 | C57BL/6NTac | |
| 17179A-D4 | VGB6 | C57BL/6NTac | |
| 17179A-B9 | VGB6 | C57BL/6NTac | |
