17181   Slc11a1     Status: ES cell colonies screened / QC positives

GeneSlc11a1
Chromosome1
Deletion Start74,385,584
Deletion End74,375,318
Deletion Size10,267
Genome Build38
MGI IDMGI:1345275
NCBI Gene ID18173
BAC ID304O22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,376,20374,376,225CGTATTTGCGAGTGCATGTGTAG
TUP74,376,22774,376,253TGTGTCCATTATAGAGCATGCGCGTGG
TUR74,376,25874,376,280TGACTCTCACAAGTTGTCCTCTG
TDF74,384,95374,384,972CGGGCTTAACCTCACCACAC
TDP74,384,97474,384,997CCTTGCCAAGTCAGTGATGGAGCC
TDR74,384,99974,385,017AGCCACTTGGAGGCACTTC

PCR Primers

PrimerSequence
SUGAAGGACCAGAAATCGGAGG
SDGAGACCCGACCTAGAACTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR65 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17181A-A4VGB6C57BL/6NTac
17181A-A7VGB6C57BL/6NTac
17181A-B5VGB6C57BL/6NTac
17181A-C12VGB6C57BL/6NTac
17181A-E9VGB6C57BL/6NTac
17181A-F6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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