| Gene | Slc19a1 |
| Chromosome | 10 |
| Deletion Start | 77,049,864 |
| Deletion End | 77,038,400 |
| Deletion Size | 11,465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,038,804 | 77,038,825 | TGGGCCTGAGGAAGAATGTAAC |
| TUP | 77,038,826 | 77,038,851 | CCAAGGAGTGCCAGCTTCTACTACCA |
| TUR | 77,038,857 | 77,038,876 | CGTGGCTGGGCATGAACAAG |
| TDF | 77,049,115 | 77,049,133 | GGCCTCTGTTGGTCACTTC |
| TDP | 77,049,136 | 77,049,157 | TGCACTGCAGGCAAGGCTATGC |
| TDR | 77,049,199 | 77,049,219 | CAGGAGGGTTCCAGGACAATG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGTGGCTGAGACTGG |
| SD | ATAGTTGAGCCCGACACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17184A-A7 | VGB6 | C57BL/6NTac | |
| 17184A-C7 | VGB6 | C57BL/6NTac | |
| 17184A-E7 | VGB6 | C57BL/6NTac | |
| 17184A-E8 | VGB6 | C57BL/6NTac | |
