| Gene | Slc1a7 |
| Chromosome | 4 |
| Deletion Start | 108,002,215 |
| Deletion End | 107,968,579 |
| Deletion Size | 33,637 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,969,315 | 107,969,334 | CAAGTGACCCTGGGCTGAAG |
| TUP | 107,969,343 | 107,969,368 | TGAGAACAGCGCTCATGGAGAGGAAA |
| TUR | 107,969,391 | 107,969,412 | TCACCACTCTGTCTCCCATTTC |
| TDF | 108,001,784 | 108,001,808 | TTAGGCGATGTTCATCTTTAGGAAG |
| TDP | 108,001,814 | 108,001,838 | CACTATGGTTTGCCGAGCTAGCCCA |
| TDR | 108,001,901 | 108,001,922 | CACTGCTTAACTGCTGGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTTTGAGGACTGGACGC |
| SD | CCTTAGTGTGGACACTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17185A-A8 | VGB6 | C57BL/6NTac | |
| 17185A-C1 | VGB6 | C57BL/6NTac | |
| 17185A-D11 | VGB6 | C57BL/6NTac | |
| 17185A-E2 | VGB6 | C57BL/6NTac | |
| 17185A-E1 | VGB6 | C57BL/6NTac | |
| 17185A-A5 | VGB6 | C57BL/6NTac | |
