| Gene | Slc24a6 |
| Chromosome | 5 |
| Deletion Start | 120,533,103 |
| Deletion End | 120,513,180 |
| Deletion Size | 19,924 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,513,514 | 120,513,538 | GGTGGTCTGTGACAAACTTATTCTG |
| TUP | 120,513,539 | 120,513,566 | TGAACCAGTCATCTTGGAGCCAGGCTTT |
| TUR | 120,513,578 | 120,513,596 | CCCGTCAAAGAGGCTTTCC |
| TDF | 120,532,123 | 120,532,144 | TGCTTCAGTGACATCATGTTGG |
| TDP | 120,532,148 | 120,532,176 | ATGTTGCACAAAGCAAGCAAACTCAGTGC |
| TDR | 120,532,214 | 120,532,232 | CTTTACCAGGCCCGACTTC |
PCR Primers
| Primer | Sequence |
| SU | AGTCACCTATGGGTTGTGGG |
| SD | CCACAGAGAGTGGAAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17186A-A11 | VGB6 | C57BL/6NTac | |
| 17186A-B7 | VGB6 | C57BL/6NTac | |
| 17186A-D3 | VGB6 | C57BL/6NTac | |
| 17186A-D5 | VGB6 | C57BL/6NTac | |
| 17186A-F7 | VGB6 | C57BL/6NTac | |
