| Gene | Slc25a4 |
| Chromosome | 8 |
| Deletion Start | 46,210,919 |
| Deletion End | 46,207,461 |
| Deletion Size | 3,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,210,476 | 46,210,498 | CAGCAAGCGACATTCCATCTAAG |
| TUP | 46,210,453 | 46,210,474 | CCTCTTAGGCGGGCCTCACCCT |
| TUR | 46,210,408 | 46,210,430 | TGTCAAGGGTGGATGCAGATAAG |
| TDF | 46,207,576 | 46,207,599 | CACTTGACTGCTGGAGGAAGATTG |
| TDP | 46,207,541 | 46,207,568 | TGAAGGAGCCAACGCTTTCTTCAAAGGT |
| TDR | 46,207,510 | 46,207,532 | CACCCATGCCTCTCAGTACATTG |
PCR Primers
| Primer | Sequence |
| SU | CTTTAAACGTCCCAAGCTGC |
| SD | ATGACCCGCTTACGTTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17187A-B7 | VGB6 | C57BL/6NTac | |
| 17187A-B9 | VGB6 | C57BL/6NTac | |
| 17187A-G6 | VGB6 | C57BL/6NTac | |
| 17187A-H12 | VGB6 | C57BL/6NTac | |
