| Gene | Slc27a4 |
| Chromosome | 2 |
| Deletion Start | 29,815,741 |
| Deletion End | 29,804,169 |
| Deletion Size | 11,573 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,804,770 | 29,804,793 | GAGGTAAGAAAGGGACGAAGTAAC |
| TUP | 29,804,795 | 29,804,821 | CCCTAAACTGTCTGTGCTATTGCTGCA |
| TUR | 29,804,834 | 29,804,855 | AGAACACAGGAGTGAGCAAGAC |
| TDF | 29,815,086 | 29,815,110 | GGAGCAATAGGTAGCATTTCAGAGG |
| TDP | 29,815,113 | 29,815,141 | AGCAGATCTAGGGAGAGGGTCTGTCATTC |
| TDR | 29,815,142 | 29,815,164 | TGGTAACCAGGATTTGAACACAG |
PCR Primers
| Primer | Sequence |
| SU | GAATGGACTGTCCTGTTTGG |
| SD | GTCTACAGGCTTTGCCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17190B-A10 | VGB6 | C57BL/6NTac | |
| 17190B-G11 | VGB6 | C57BL/6NTac | |
