| Gene | Slc4a1 |
| Chromosome | 11 |
| Deletion Start | 102,361,392 |
| Deletion End | 102,350,270 |
| Deletion Size | 11,123 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,360,906 | 102,360,924 | GACAGGGCAGCCAGGTATG |
| TUP | 102,360,883 | 102,360,905 | TCTGGGACAAGGACACAGACCCA |
| TUR | 102,360,836 | 102,360,855 | CCTGCTGCTTGGCCTCAATG |
| TDF | 102,350,652 | 102,350,674 | TGACAGGGTGAAAGGCTAATGAG |
| TDP | 102,350,623 | 102,350,650 | CGCACAGGGTGCATCTCTTGTATCATCA |
| TDR | 102,350,596 | 102,350,618 | CCACACCGAATGTCCCAAATAAC |
PCR Primers
| Primer | Sequence |
| SU | ATCATGTTCTGGGAGTTGGC |
| SD | AAGTGTCCTCCGTCTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17193A-A6 | VGB6 | C57BL/6NTac | |
| 17193A-F2 | VGB6 | C57BL/6NTac | |
| 17193A-F5 | VGB6 | C57BL/6NTac | |
| 17193A-F8 | VGB6 | C57BL/6NTac | |
| 17193A-H5 | VGB6 | C57BL/6NTac | |
