| Gene | Slc7a6 |
| Chromosome | 8 |
| Deletion Start | 106,196,681 |
| Deletion End | 106,179,141 |
| Deletion Size | 17,541 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,179,391 | 106,179,411 | CCATTGGTGGGCTCTTCTCTG |
| TUP | 106,179,413 | 106,179,434 | CGTGGGTGCCCTTTGCTATGCA |
| TUR | 106,179,443 | 106,179,464 | CCCTGATTTGGTGATGGTGGTC |
| TDF | 106,196,039 | 106,196,061 | GAGTGTCTCTTTGCTCCCATTTG |
| TDP | 106,196,063 | 106,196,087 | CCCACTGTTGGAGGCTGAGTGCTTA |
| TDR | 106,196,089 | 106,196,108 | GCCTCTGTGTAGCCCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGGCCTCTTTGCTCAGTAAG |
| SD | CTTGGTCAGAGGGTCAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17195A-H8 | VGB6 | C57BL/6NTac | |
| 17195A-C6 | VGB6 | C57BL/6NTac | |
| 17195A-F6 | VGB6 | C57BL/6NTac | |
| 17195A-E11 | VGB6 | C57BL/6NTac | |
| 17195A-F3 | VGB6 | C57BL/6NTac | |
| 17195A-C4 | VGB6 | C57BL/6NTac | |
