| Gene | Slco1c1 |
| Chromosome | 6 |
| Deletion Start | 141,569,614 |
| Deletion End | 141,531,481 |
| Deletion Size | 38,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,531,538 | 141,531,558 | GGAGGGCCATCCTTTACAGTC |
| TUP | 141,531,569 | 141,531,590 | CCACAGAGGAAGCGCGGCCATG |
| TUR | 141,531,607 | 141,531,627 | AAACGACACCCAACCCTCTAC |
| TDF | 141,569,006 | 141,569,028 | TGGGACAGCAATGGGCTAAATAG |
| TDP | 141,569,031 | 141,569,056 | CTGCCTCAGCAAGCAGGAAGATGGCT |
| TDR | 141,569,137 | 141,569,154 | GCCAGCAGAGGCAACTTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAATCTGTGATGGTGTC |
| SD | CTGTATCAGTTCAAGGAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 149 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17197A-G10 | VGB6 | C57BL/6NTac | |
| 17197A-F10 | VGB6 | C57BL/6NTac | |
| 17197A-B11 | VGB6 | C57BL/6NTac | |
| 17197A-A7 | VGB6 | C57BL/6NTac | |
