| Gene | Sparc |
| Chromosome | 11 |
| Deletion Start | 55,409,981 |
| Deletion End | 55,395,509 |
| Deletion Size | 14,473 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,409,584 | 55,409,605 | GAGCATGTGCAGAAGACTCAAG |
| TUP | 55,409,552 | 55,409,576 | CCCAGAACCCAAATGGTGGCTTACA |
| TUR | 55,409,520 | 55,409,541 | GAGGTCAAGAAGAGGCCATCAG |
| TDF | 55,396,042 | 55,396,063 | CATGGCAGAGCTGTGGGTAAAG |
| TDP | 55,396,013 | 55,396,035 | TCCCAGCCCTGGGCTCCTACTAC |
| TDR | 55,395,982 | 55,396,004 | TGGGAGAACAACAGTCCAGTATG |
PCR Primers
| Primer | Sequence |
| SU | TCCTCAATAGAAGGAAACTGGG |
| SD | CTGCCTAACTCCAAACCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17199B-F11 | VGB6 | C57BL/6NTac | |
| 17199C-A1 | VGB6 | C57BL/6NTac | |
| 17199C-D12 | VGB6 | C57BL/6NTac | |
| 17199C-E6 | VGB6 | C57BL/6NTac | |
| 17199C-F5 | VGB6 | C57BL/6NTac | |
| 17199C-H5 | VGB6 | C57BL/6NTac | |
