| Gene | Timp3 |
| Chromosome | 10 |
| Deletion Start | 86,338,450 |
| Deletion End | 86,300,857 |
| Deletion Size | 37,594 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,300,925 | 86,300,944 | CACATGCTCTCCCAGCCATC |
| TUP | 86,300,946 | 86,300,969 | CCAGGATGCCTTCTGCAACTCCGA |
| TUR | 86,300,987 | 86,301,007 | GCCCAGGGAAACTGAGTCTTC |
| TDF | 86,337,737 | 86,337,761 | TGCCTTAATCATCTCAGTCAAAGTC |
| TDP | 86,337,763 | 86,337,787 | CACTAGGGAGTACCAGACGTTGCCA |
| TDR | 86,337,791 | 86,337,812 | TGTTGTCTCCAGACATGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATATCACTCTGCAGCCCTGG |
| SD | AGCTATTGCTGTCTGGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17200A-D2 | VGB6 | C57BL/6NTac | |
| 17200A-E2 | VGB6 | C57BL/6NTac | |
| 17200A-E3 | VGB6 | C57BL/6NTac | |
| 17200A-G11 | VGB6 | C57BL/6NTac | |
| 17200A-G12 | VGB6 | C57BL/6NTac | |
