| Gene | Tmc8 |
| Chromosome | 11 |
| Deletion Start | 117,792,814 |
| Deletion End | 117,783,113 |
| Deletion Size | 9,702 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,783,994 | 117,784,011 | ATGCCACCCAGCACAAAC |
| TUP | 117,784,012 | 117,784,038 | TCAGACATCCTCGGTATAGGGTCGAGA |
| TUR | 117,784,041 | 117,784,062 | ACACTCTATCTCCCAACTCCAC |
| TDF | 117,792,156 | 117,792,176 | CAACTGGTGTGGGTGAGTGTG |
| TDP | 117,792,177 | 117,792,202 | CATCGGCCTGACCCTTGAGGAATGTG |
| TDR | 117,792,217 | 117,792,235 | CCTGTCAGCTCCGTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGTTTAGGACAGACAGCC |
| SD | CTTGCAGCCTCATGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17202A-A12 | VGB6 | C57BL/6NTac | |
| 17202A-C1 | VGB6 | C57BL/6NTac | |
| 17202A-D12 | VGB6 | C57BL/6NTac | |
| 17202A-F12 | VGB6 | C57BL/6NTac | |
| 17202A-H9 | VGB6 | C57BL/6NTac | |
