| Gene | Tmem120a |
| Chromosome | 5 |
| Deletion Start | 135,744,125 |
| Deletion End | 135,735,590 |
| Deletion Size | 8,536 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,743,253 | 135,743,276 | GGCCAATAGGAGACTAGGTTAGAC |
| TUP | 135,743,223 | 135,743,249 | TGCCTCTATTGGACTGTTGCCTAGCCT |
| TUR | 135,743,151 | 135,743,171 | GGGAGACATGGAGCTGTGTAG |
| TDF | 135,736,357 | 135,736,374 | GCTCACCGTGCTGCTTTG |
| TDP | 135,736,332 | 135,736,356 | TGCCACCTAGGCTTTGTGCAATTCC |
| TDR | 135,736,303 | 135,736,321 | CAGGCACCCGCTCTGATAG |
PCR Primers
| Primer | Sequence |
| SU | ACGTGTCTCCTTGGTTTGTG |
| SD | TGGCCCTTGGAATAAAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17203A-A9 | VGB6 | C57BL/6NTac | |
| 17203A-D5 | VGB6 | C57BL/6NTac | |
| 17203A-G2 | VGB6 | C57BL/6NTac | |
| 17203A-H4 | VGB6 | C57BL/6NTac | |
| 17203A-H10 | VGB6 | C57BL/6NTac | |
