| Gene | Tmem130 |
| Chromosome | 5 |
| Deletion Start | 144,761,529 |
| Deletion End | 144,737,284 |
| Deletion Size | 24,246 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 144,761,448 | 144,761,465 | TGGCGGCAGGTAAGTCAG |
| TUP | 144,761,428 | 144,761,447 | AGCCTGCCTGGAGCTGTCCA |
| TUR | 144,761,383 | 144,761,401 | CAGAACGCACCGAGTCATC |
| TDF | 144,737,804 | 144,737,823 | CCCAGAAGGACATCGTGGAG |
| TDP | 144,737,774 | 144,737,801 | AGGTGCTTTGTAGATGGAGGCTAGACCA |
| TDR | 144,737,725 | 144,737,743 | GATGCCCTGGTCTGCCTAC |
PCR Primers
| Primer | Sequence |
| SU | TGGAAAGATGGCTCTGGG |
| SD | TGTGACCTCCTTCCTCGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17206A-H9 | VGB6 | C57BL/6NTac | |
| 17206A-C1 | VGB6 | C57BL/6NTac | |
