| Gene | Tmem136 |
| Chromosome | 9 |
| Deletion Start | 43,113,656 |
| Deletion End | 43,111,240 |
| Deletion Size | 2,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,113,595 | 43,113,617 | GGCTGGCTCTCACTCTATACATC |
| TUP | 43,113,571 | 43,113,594 | TTTCTGCTGCCTGAACAAGCACCG |
| TUR | 43,113,547 | 43,113,567 | AGCCGACAGCTCCACTCACAG |
| TDF | 43,111,700 | 43,111,722 | GATCATCATGGCCCTAGCACTTG |
| TDP | 43,111,671 | 43,111,696 | AGTCGGGCACAGAGGTCAATGCTGTA |
| TDR | 43,111,632 | 43,111,650 | CATCTGGAGCAGCGGGTTG |
PCR Primers
| Primer | Sequence |
| SU | GCAATGGAACTCGTGAAGTG |
| SD | CCTGAGAGTCCCTCCCTTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17207A-H1 | VGB6 | C57BL/6NTac | |
| 17207A-D4 | VGB6 | C57BL/6NTac | |
| 17207A-B5 | VGB6 | C57BL/6NTac | |
