| Gene | Tmem167 |
| Chromosome | 13 |
| Deletion Start | 90,104,456 |
| Deletion End | 90,089,784 |
| Deletion Size | 14,673 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,090,023 | 90,090,044 | ACTTGCTGCGTTGTTTAGTGTG |
| TUP | 90,090,049 | 90,090,072 | TACGGTGGCATTCCACTAAGCCGA |
| TUR | 90,090,112 | 90,090,132 | AGACTGAGACTCCAGCGTTTG |
| TDF | 90,104,134 | 90,104,157 | CTGAAGTTGTCCTCTAACCTCCAC |
| TDP | 90,104,161 | 90,104,188 | TGCACTGTGGCATGATCACTCACATGAA |
| TDR | 90,104,209 | 90,104,231 | GGGATCTAACCCAAATCCAAACC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCTGGAAAGACACCACAG |
| SD | CTGGGTTTCTACTTCGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17209A-C12 | VGB6 | C57BL/6NTac | |
| 17209A-G2 | VGB6 | C57BL/6NTac | |
| 17209A-G11 | VGB6 | C57BL/6NTac | |
| 17209A-H5 | VGB6 | C57BL/6NTac | |
