| Gene | Tmem167b |
| Chromosome | 3 |
| Deletion Start | 108,562,277 |
| Deletion End | 108,558,756 |
| Deletion Size | 3,522 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,562,106 | 108,562,126 | GGATGCTCCTTGCAGTTTCTC |
| TUP | 108,562,075 | 108,562,100 | TCAAGCCCATCAAGGTACCCTAGTGC |
| TUR | 108,562,050 | 108,562,072 | GGGTGATAACGAAAAGTCAGGTG |
| TDF | 108,559,083 | 108,559,103 | GCCTAGAGCATAAGGGTGTTC |
| TDP | 108,559,046 | 108,559,074 | CATTTCCAGACTCTCGAAACCTGTGGTTC |
| TDR | 108,559,014 | 108,559,033 | TGCTTCCAAACCACGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TTAGACAGGAGCAAGCAGGC |
| SD | TGGGAAGTCTGAACAAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17210A-B9 | VGB6 | C57BL/6NTac | |
| 17210A-C6 | VGB6 | C57BL/6NTac | |
| 17210A-C9 | VGB6 | C57BL/6NTac | |
| 17210A-D2 | VGB6 | C57BL/6NTac | |
| 17210A-E4 | VGB6 | C57BL/6NTac | |
| 17210A-F9 | VGB6 | C57BL/6NTac | |
