| Gene | Tmem170 |
| Chromosome | 8 |
| Deletion Start | 111,876,632 |
| Deletion End | 111,866,286 |
| Deletion Size | 10,347 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,875,965 | 111,875,987 | AAAGAGGATGACGGGTGGGATTG |
| TUP | 111,875,939 | 111,875,964 | CTGACTGCAACCTTCGACCAGACACC |
| TUR | 111,875,912 | 111,875,931 | GTCGCTGCCCAGTAGATGAG |
| TDF | 111,867,151 | 111,867,172 | TGGGTGTAGAAGATGCGATAGG |
| TDP | 111,867,128 | 111,867,149 | TCTCTGGGTCCGCACCAGTGTT |
| TDR | 111,867,108 | 111,867,126 | GAACAGGAGCCCAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | GCAGAAGAAACTTGCCACTAG |
| SD | TGACAGTGGAACCAAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17211A-E5 | VGB6 | C57BL/6NTac | |
| 17211A-B6 | VGB6 | C57BL/6NTac | |
| 17211A-E12 | VGB6 | C57BL/6NTac | |
| 17211A-D2 | VGB6 | C57BL/6NTac | |
| 17211A-B2 | VGB6 | C57BL/6NTac | |
| 17211A-C6 | VGB6 | C57BL/6NTac | |
