| Gene | Tmem177 |
| Chromosome | 1 |
| Deletion Start | 119,910,944 |
| Deletion End | 119,909,973 |
| Deletion Size | 972 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,910,775 | 119,910,794 | GGCCAACCAGCTCCTCTTTC |
| TUP | 119,910,750 | 119,910,774 | CCCTCATCTGTGGAGCCTCTTCCAA |
| TUR | 119,910,716 | 119,910,735 | GACCCGAAGGGACACCTATG |
| TDF | 119,910,034 | 119,910,054 | ACAACAGATGTGGAGGGCAAC |
| TDP | 119,910,012 | 119,910,033 | AGTCAGCCCAGGCCGCTTCTGA |
| TDR | 119,909,975 | 119,909,994 | GCCTATGCAAGCCAGGACTG |
PCR Primers
| Primer | Sequence |
| SU | AATACAGCCATTGCCAGAGG |
| SD | GTCTCCTCACCTTGTCCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17212A-B6 | VGB6 | C57BL/6NTac | |
| 17212A-B11 | VGB6 | C57BL/6NTac | |
| 17212A-D11 | VGB6 | C57BL/6NTac | |
