| Gene | Tmem183a |
| Chromosome | 1 |
| Deletion Start | 134,361,867 |
| Deletion End | 134,347,994 |
| Deletion Size | 13,874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,361,626 | 134,361,645 | TTGGCGCTTTGCTCTGTTTC |
| TUP | 134,361,603 | 134,361,625 | AGTGACCGTGGCGGACTATGCCA |
| TUR | 134,361,566 | 134,361,583 | TTGACCCGTCCAGACCTC |
| TDF | 134,348,084 | 134,348,103 | GTGAACAGGGTGTGCAAGTC |
| TDP | 134,348,063 | 134,348,082 | TCCTGGACCCGGTGCACAGT |
| TDR | 134,348,012 | 134,348,033 | TGCTCTCAGGGAGAATGGATAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGAAGTGGACCACGATG |
| SD | GAGGAGCAGCAACATTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17213A-A8 | VGB6 | C57BL/6NTac | |
| 17213A-D11 | VGB6 | C57BL/6NTac | |
| 17213A-E7 | VGB6 | C57BL/6NTac | |
| 17213A-H6 | VGB6 | C57BL/6NTac | |
