| Gene | Tmem209 |
| Chromosome | 6 |
| Deletion Start | 30,509,684 |
| Deletion End | 30,482,884 |
| Deletion Size | 26,801 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,509,394 | 30,509,415 | GGAGCTGCCCTCTCAGTGATTG |
| TUP | 30,509,365 | 30,509,390 | AGCTGAGCCTAGCGAGACAAAGGAGA |
| TUR | 30,509,332 | 30,509,353 | ACGTGCGTTCAGTTTGGGTTAC |
| TDF | 30,483,099 | 30,483,118 | ACAGGCTGCATTGCTGAGTC |
| TDP | 30,483,068 | 30,483,095 | TCAGCTGCCACCAGTTAAACAGATGTCC |
| TDR | 30,483,010 | 30,483,033 | AGTAGAATTGCTTGCCGAAGTATC |
PCR Primers
| Primer | Sequence |
| SU | GGTCAAACCAATTGAGGGAG |
| SD | GGCTGACTTGTAGAACACTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17215A-B4 | VGB6 | C57BL/6NTac | |
| 17215A-B10 | VGB6 | C57BL/6NTac | |
| 17215A-C10 | VGB6 | C57BL/6NTac | |
| 17215A-E5 | VGB6 | C57BL/6NTac | |
| 17215A-E8 | VGB6 | C57BL/6NTac | |
| 17215A-H3 | VGB6 | C57BL/6NTac | |
