| Gene | Tmem218 |
| Chromosome | 9 |
| Deletion Start | 37,222,672 |
| Deletion End | 37,218,270 |
| Deletion Size | 4,403 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,219,119 | 37,219,143 | TCCAGTTAGAAGTCAGCACAGTTAG |
| TUP | 37,219,144 | 37,219,168 | AACTAGACTGTCCGCAGTGCAGTGC |
| TUR | 37,219,188 | 37,219,208 | GGAGAACCTGGGAGCAAAGAG |
| TDF | 37,222,545 | 37,222,564 | GGCCTCTTCCTGCTTCTTAC |
| TDP | 37,222,566 | 37,222,593 | CATCATTTGCTGGAGCCAATCTATGCCA |
| TDR | 37,222,615 | 37,222,637 | TGTCCCGTGTTTCCGTTACTATG |
PCR Primers
| Primer | Sequence |
| SU | CAATAGGCTGAGCAGAAGGC |
| SD | TTCTGTTGGATAACAAAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17216B-E7 | VGB6 | C57BL/6NTac | |
| 17216B-E12 | VGB6 | C57BL/6NTac | |
