| Gene | Tmem44 |
| Chromosome | 16 |
| Deletion Start | 30,550,496 |
| Deletion End | 30,545,130 |
| Deletion Size | 5,367 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,549,755 | 30,549,774 | TGTAGCCTGGGTTCTGTGAG |
| TUP | 30,549,730 | 30,549,754 | AGCATAGCCTCCAGGTCTTCAGCCA |
| TUR | 30,549,686 | 30,549,706 | CCACCCTGGAAACTGTCTCTG |
| TDF | 30,545,447 | 30,545,465 | GATGCAGAGGGATGGGTTC |
| TDP | 30,545,424 | 30,545,445 | CAGCCCGTCTCTACTGCCTGGC |
| TDR | 30,545,391 | 30,545,409 | TGGGTCAGGAGACAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCAGAGGCGATGTGTACC |
| SD | TTGTTTGCTTATCTATGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17218A-B3 | VGB6 | C57BL/6NTac | |
| 17218A-D5 | VGB6 | C57BL/6NTac | |
